{"id":340814,"date":"2024-10-19T23:53:02","date_gmt":"2024-10-19T23:53:02","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-cen-iso-ts-179192013\/"},"modified":"2024-10-25T23:03:49","modified_gmt":"2024-10-25T23:03:49","slug":"bsi-pd-cen-iso-ts-179192013","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-cen-iso-ts-179192013\/","title":{"rendered":"BSI PD CEN ISO\/TS 17919:2013"},"content":{"rendered":"

This Technical Specification specifies a horizontal method for the molecular detection of clostridia carrying botulinum neurotoxin A, B, E, and F genes by a PCR method. This method detects the genes and not the toxins, therefore a positive result does not necessarily mean the presence of these toxins in the sample investigated. This Technical Specification is applicable to products for human consumption, animal feed, and environmental samples.<\/p>\n

The PCR assays for detection of genetic sequences encoding specific toxin types are described in Annexes B and C.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
4<\/td>\nForeword <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\nSection sec_1
Section sec_2
Section sec_3
Section sec_4
Section sec_4.1
1\tScope
2\tNormative references
3\tTerms and definitions
4\tSymbols and abbreviated terms
4.1\tSymbols <\/td>\n<\/tr>\n
10<\/td>\nSection sec_4.2
Section sec_5
Section sec_5.1
Section sec_5.2
Section sec_5.3
Section sec_5.4
Section sec_5.5
Section sec_5.6
4.2\tAbbreviated terms
5\tPrinciple
5.1\tGeneral
5.2\tMicrobial enrichment
5.3\tNucleic acid extraction
5.4\tAmplification by PCR
5.5\tDetection of PCR products
5.6\tConfirmation <\/td>\n<\/tr>\n
11<\/td>\nSection sec_6
Section sec_6.1
Section sec_6.2
Section sec_6.2.1
Section sec_6.2.2
Section sec_6.2.3
Section sec_6.2.3.1
Section sec_6.2.3.2
Section sec_6.2.3.3
Section sec_6.2.4
Section sec_6.2.4.1
Section sec_6.2.4.1.1
6\tReagents
6.1\tGeneral
6.2\tCulture media <\/td>\n<\/tr>\n
12<\/td>\nSection sec_6.2.4.1.2
Section sec_6.2.4.1.3
Section sec_6.2.4.2
Section sec_6.3
Section sec_6.3.1
Section sec_6.3.2
Section sec_6.3.3
Section sec_6.3.4
Section sec_6.3.5
Section sec_6.3.6
Section sec_6.3.7
Section sec_6.3.8
Section sec_6.3.9
Section sec_6.3.10
Section sec_6.3.11
Section sec_6.3.12
Section sec_6.3.13
6.3\tNucleic acid extraction <\/td>\n<\/tr>\n
13<\/td>\nSection sec_6.3.14
Section sec_6.3.15
Section sec_6.3.16
Section sec_6.4
Section sec_6.4.1
Section sec_6.4.2
Section sec_6.4.3
Section sec_6.4.4
Section sec_7
Section sec_7.1
Section sec_7.2
Section sec_7.2.1
Section sec_7.2.2
Section sec_7.2.3
Section sec_7.2.4
Section sec_7.3
Section sec_7.3.1
Section sec_7.3.2
Section sec_7.3.3
6.4\tReagents for PCR
7\tApparatus and equipment
7.1\tGeneral
7.2\tEquipment for sample preparation prior to enrichment
7.3\tEquipment for microbial enrichment <\/td>\n<\/tr>\n
14<\/td>\nSection sec_7.3.4
Section sec_7.4
Section sec_7.4.1
Section sec_7.4.2
Section sec_7.4.3
Section sec_7.4.4
Section sec_7.4.5
Section sec_7.5
Section sec_7.5.1
Section sec_7.5.2
Section sec_7.5.3
Section sec_7.5.4
Section sec_7.6
Section sec_7.6.1
Section sec_7.6.1.1
Section sec_7.6.1.2
Section sec_7.6.1.3
Section sec_7.6.1.4
Section sec_7.6.2
Section sec_7.6.2.1
Section sec_7.6.2.2
7.4\tEquipment used for nucleic acid extraction
7.5\tEquipment used for PCR
7.6\tEquipment used for the detection of the PCR product <\/td>\n<\/tr>\n
15<\/td>\nSection sec_8
Section sec_9
Section sec_9.1
Section sec_9.1.1
Section sec_9.1.2
Section sec_9.1.3
Section sec_9.2
Section sec_9.2.1
Section sec_9.2.1.1
Section sec_9.2.1.2
Section sec_9.2.1.2.1
8\tSampling
9\tProcedure
9.1\tSample preparation prior to enrichment
9.2\tMicrobial enrichment <\/td>\n<\/tr>\n
16<\/td>\nSection sec_9.2.1.2.2
Section sec_9.2.1.3
Section sec_9.2.2
Section sec_9.2.3
Section sec_9.3
Section sec_9.3.1
Section sec_9.3.2
9.3\tNucleic acid preparation <\/td>\n<\/tr>\n
17<\/td>\nSection sec_9.3.3
Section sec_9.3.4
Section sec_9.4
Section sec_9.4.1
Section sec_9.4.2
Section sec_9.5
Section sec_9.5.1
9.4\tPCR amplification
9.5\tConfirmation of a positive PCR result <\/td>\n<\/tr>\n
18<\/td>\nAnnex sec_A
Figure fig_A.1
Annex\u00a0A
\n(normative)<\/p>\n

Flow-diagram of the procedure <\/td>\n<\/tr>\n

19<\/td>\nAnnex sec_B
Annex sec_B.1
Annex sec_B.1.1
Annex sec_B.1.2
Annex sec_B.1.2.1
Annex sec_B.1.2.2
Annex sec_B.1.2.3
Annex sec_B.1.2.3.1
Annex\u00a0B
\n(informative)<\/p>\n

Multiplex PCR assays for detection of genes encoding botulinum neurotoxin types A, B, E, and F using agarose gel electrophoresis <\/td>\n<\/tr>\n

20<\/td>\nTable tab_B.1
Annex sec_B.1.2.3.2 <\/td>\n<\/tr>\n
21<\/td>\nTable tab_B.2
Annex sec_B.1.2.4
Annex sec_B.1.2.4.1
Annex sec_B.1.2.4.2
Annex sec_B.1.2.5 <\/td>\n<\/tr>\n
22<\/td>\nAnnex sec_B.1.2.6
Annex sec_B.1.3
Annex sec_B.1.4
Annex sec_B.1.4.1
Annex sec_B.1.4.2
Annex sec_B.1.4.2.1
Annex sec_B.1.4.2.2
Annex sec_B.1.4.2.3
Annex sec_B.1.4.2.4
Annex sec_B.1.4.2.5
Annex sec_B.1.4.2.6 <\/td>\n<\/tr>\n
23<\/td>\nTable tab_B.3
Annex sec_B.1.4.3
Annex sec_B.1.4.3.1
Annex sec_B.1.4.3.2
Annex sec_B.1.4.3.3
Annex sec_B.1.4.3.4
Annex sec_B.1.4.3.5
Annex sec_B.1.4.3.6
Annex sec_B.1.4.3.7
Annex sec_B.1.4.3.8
Annex sec_B.1.4.3.9
Annex sec_B.1.4.3.10
Annex sec_B.1.4.3.11
Annex sec_B.1.4.3.12
Annex sec_B.1.4.3.13
Annex sec_B.1.4.3.14 <\/td>\n<\/tr>\n
24<\/td>\nAnnex sec_B.1.4.3.15
Annex sec_B.1.4.3.16
Annex sec_B.1.4.3.17
Annex sec_B.1.5
Annex sec_B.1.5.1
Annex sec_B.1.5.2
Annex sec_B.1.5.2.1
Annex sec_B.1.5.2.2
Annex sec_B.1.5.2.3
Annex sec_B.1.5.2.4
Annex sec_B.1.5.2.5
Annex sec_B.1.5.2.6
Annex sec_B.1.5.2.7
Annex sec_B.1.5.2.8
Annex sec_B.1.5.2.9
Annex sec_B.1.5.2.10 <\/td>\n<\/tr>\n
25<\/td>\nAnnex sec_B.1.6
Annex sec_B.1.6.1
Annex sec_B.1.6.2
Annex sec_B.1.6.2.1
Annex sec_B.1.6.2.2
Annex sec_B.1.6.2.3
Annex sec_B.1.6.2.4
Annex sec_B.1.6.2.5
Annex sec_B.1.6.2.6
Annex sec_B.1.6.2.7
Annex sec_B.1.6.2.8
Annex sec_B.1.6.2.9
Table tab_B.4
Annex sec_B.1.6.3 <\/td>\n<\/tr>\n
26<\/td>\nTable tab_B.5
Annex sec_B.1.6.4
Table tab_B.6
Annex sec_B.1.6.5
Annex sec_B.1.7
Annex sec_B.1.7.1 <\/td>\n<\/tr>\n
27<\/td>\nTable tab_B.7
Annex sec_B.1.7.2
Annex sec_B.1.7.2.1
Annex sec_B.1.7.2.2
Annex sec_B.1.7.2.3
Annex sec_B.1.7.2.4
Annex sec_B.1.7.3 <\/td>\n<\/tr>\n
28<\/td>\nTable tab_B.8
Annex sec_B.1.7.4
Annex sec_B.1.7.4.1
Annex sec_B.1.7.4.2
Annex sec_B.1.7.4.3
Annex sec_B.1.7.4.4 <\/td>\n<\/tr>\n
29<\/td>\nAnnex sec_B.1.7.4.5
Table tab_B.9
Annex sec_B.1.7.5
Annex sec_B.1.7.6
Annex sec_B.2
Annex sec_B.2.1
Annex sec_B.2.2
Annex sec_B.2.2.1 <\/td>\n<\/tr>\n
30<\/td>\nAnnex sec_B.2.2.2
Annex sec_B.2.2.3
Annex sec_B.2.2.3.1
Table tab_B.10
Annex sec_B.2.2.3.2 <\/td>\n<\/tr>\n
31<\/td>\nTable tab_B.11
Annex sec_B.2.2.4
Annex sec_B.2.2.5
Annex sec_B.2.2.6 <\/td>\n<\/tr>\n
32<\/td>\nAnnex sec_B.2.3
Annex sec_B.2.4
Annex sec_B.2.4.1
Annex sec_B.2.4.2
Annex sec_B.2.4.2.1
Annex sec_B.2.4.2.2
Annex sec_B.2.4.2.3
Annex sec_B.2.4.2.4
Annex sec_B.2.4.2.5
Annex sec_B.2.4.2.6
Table tab_B.12
Annex sec_B.2.4.3
Annex sec_B.2.5 <\/td>\n<\/tr>\n
33<\/td>\nAnnex sec_B.2.6
Annex sec_B.2.6.1
Annex sec_B.2.6.2
Annex sec_B.2.6.2.1
Annex sec_B.2.6.2.2
Annex sec_B.2.6.2.3
Annex sec_B.2.6.2.4
Annex sec_B.2.6.2.5
Annex sec_B.2.6.2.6
Annex sec_B.2.6.2.7
Annex sec_B.2.6.2.8
Annex sec_B.2.6.2.9
Table tab_B.13
Annex sec_B.2.6.3 <\/td>\n<\/tr>\n
34<\/td>\nTable tab_B.14
Annex sec_B.2.6.4
Table tab_B.15
Annex sec_B.2.6.5
Annex sec_B.2.7
Annex sec_B.2.7.1 <\/td>\n<\/tr>\n
35<\/td>\nTable tab_B.16
Annex sec_B.2.7.2
Annex sec_B.2.7.2.1
Annex sec_B.2.7.2.2
Annex sec_B.2.7.2.3
Annex sec_B.2.7.2.4
Annex sec_B.2.7.3
Table tab_B.17 <\/td>\n<\/tr>\n
36<\/td>\nAnnex sec_B.2.7.4
Annex sec_B.2.7.4.1
Annex sec_B.2.7.4.2
Table tab_B.18
Annex sec_B.2.7.5
Annex sec_B.2.7.6 <\/td>\n<\/tr>\n
37<\/td>\nAnnex sec_C
Annex sec_C.1
Annex sec_C.1.1
Annex sec_C.1.2
Annex sec_C.1.2.1
Annex sec_C.1.2.2
Annex sec_C.1.2.3
Annex sec_C.1.2.3.1
Annex\u00a0C
\n(informative)<\/p>\n

Assays for detection of genes encoding botulinum neurotoxin types A, B, E, and F using real-time PCR <\/td>\n<\/tr>\n

38<\/td>\nTable tab_C.1
Annex sec_C.1.2.3.2
Table tab_C.2
Annex sec_C.1.2.4
Annex sec_C.1.2.4.1 <\/td>\n<\/tr>\n
39<\/td>\nAnnex sec_C.1.2.4.2
Annex sec_C.1.2.5
Annex sec_C.1.2.6
Annex sec_C.1.3
Annex sec_C.1.4
Annex sec_C.1.4.1
Annex sec_C.1.4.2
Annex sec_C.1.4.3
Annex sec_C.1.4.4 <\/td>\n<\/tr>\n
40<\/td>\nAnnex sec_C.1.4.5
Table tab_C.3
Annex sec_C.1.5
Annex sec_C.1.5.1
Annex sec_C.1.5.2
Annex sec_C.1.5.2.1
Annex sec_C.1.5.2.2
Annex sec_C.1.5.2.3
Annex sec_C.1.5.2.4 <\/td>\n<\/tr>\n
41<\/td>\nAnnex sec_C.1.6
Annex sec_C.1.6.1
Table tab_C.4
Table tab_C.5
Annex sec_C.1.6.2
Annex sec_C.1.6.2.1
Annex sec_C.1.6.2.2 <\/td>\n<\/tr>\n
42<\/td>\nAnnex sec_C.1.6.2.3
Annex sec_C.1.6.2.4
Annex sec_C.1.6.3
Table tab_C.6
Annex sec_C.1.6.4
Annex sec_C.1.6.5
Annex sec_C.2
Annex sec_C.2.1
Annex sec_C.2.2
Annex sec_C.2.2.1 <\/td>\n<\/tr>\n
43<\/td>\nAnnex sec_C.2.2.2
Annex sec_C.2.2.3
Annex sec_C.2.2.3.1
Table tab_C.7
Annex sec_C.2.2.3.2
Table tab_C.8
Annex sec_C.2.2.4
Annex sec_C.2.2.4.1
Annex sec_C.2.2.4.2 <\/td>\n<\/tr>\n
44<\/td>\nAnnex sec_C.2.2.4.3
Annex sec_C.2.2.5
Annex sec_C.2.2.6
Annex sec_C.2.3
Annex sec_C.2.4
Annex sec_C.2.4.1
Annex sec_C.2.4.2
Annex sec_C.2.4.3
Annex sec_C.2.4.4 <\/td>\n<\/tr>\n
45<\/td>\nTable tab_C.9
Annex sec_C.2.4.5
Annex sec_C.2.4.5.1
Annex sec_C.2.4.5.2
Annex sec_C.2.4.5.3
Annex sec_C.2.5
Annex sec_C.2.5.1 <\/td>\n<\/tr>\n
46<\/td>\nAnnex sec_C.2.5.2
Annex sec_C.2.5.2.1
Annex sec_C.2.5.2.2
Annex sec_C.2.5.2.3
Annex sec_C.2.5.2.4
Annex sec_C.2.6
Annex sec_C.2.6.1
Table tab_C.10
Table tab_C.11 <\/td>\n<\/tr>\n
47<\/td>\nAnnex sec_C.2.6.2
Annex sec_C.2.6.2.1
Annex sec_C.2.6.2.2
Annex sec_C.2.6.2.3
Annex sec_C.2.6.2.4
Annex sec_C.2.6.3
Table tab_C.12
Annex sec_C.2.6.4
Annex sec_C.2.6.5 <\/td>\n<\/tr>\n
48<\/td>\nAnnex sec_D
Annex sec_D.1
Annex sec_D.2
Annex sec_D.2.1
Annex sec_D.2.2
Annex sec_D.2.2.1
Annex sec_D.2.2.1.1
Annex sec_D.2.2.1.2
Annex sec_D.2.2.2
Annex sec_D.2.2.2.1
Annex\u00a0D
\n(informative)<\/p>\n

Preparation of C. botulinum spores <\/td>\n<\/tr>\n

49<\/td>\nAnnex sec_D.2.2.2.2
Annex sec_D.2.2.3
Annex sec_D.2.3
Annex sec_D.2.3.1
Annex sec_D.2.3.1.1
Annex sec_D.2.3.1.2
Annex sec_D.2.3.2
Annex sec_D.2.3.3 <\/td>\n<\/tr>\n
50<\/td>\nAnnex sec_D.3
Annex sec_D.3.1
Annex sec_D.3.1.1
Annex sec_D.3.1.2
Annex sec_D.3.2
Annex sec_D.3.2.1
Annex sec_D.3.2.2
Annex sec_D.3.2.3
Annex sec_D.3.2.4
Annex sec_D.3.2.5
Annex sec_D.3.2.6
Annex sec_D.3.2.7
Annex sec_D.3.2.8
Annex sec_D.3.2.9
Annex sec_D.3.2.10
Annex sec_D.3.2.11
Annex sec_D.4
Annex sec_D.4.1
Annex sec_D.4.2
Annex sec_D.4.2.1
Annex sec_D.4.2.2
Annex sec_D.4.2.3 <\/td>\n<\/tr>\n
51<\/td>\nAnnex sec_D.4.2.4
Annex sec_D.4.3
Annex sec_D.4.3.1
Annex sec_D.4.3.2
Annex sec_D.4.3.3
Annex sec_D.4.3.4
Annex sec_D.5
Annex sec_D.5.1
Annex sec_D.5.1.1
Annex sec_D.5.1.2
Annex sec_D.5.2
Annex sec_D.5.3 <\/td>\n<\/tr>\n
52<\/td>\nAnnex sec_D.5.4
Annex sec_D.5.4.1
Annex sec_D.5.4.2
Annex sec_D.5.4.2.1
Annex sec_D.5.4.2.2
Annex sec_D.5.4.2.3 <\/td>\n<\/tr>\n
53<\/td>\nFigure fig_D.1 <\/td>\n<\/tr>\n
54<\/td>\nReference ref_1
Reference ref_2
Reference ref_3
Reference ref_4
Reference ref_5
Reference ref_6
Reference ref_7
Reference ref_8
Reference ref_9
Reference ref_10
Reference ref_11
Reference ref_12
Reference ref_13
Bibliography
Bibliography <\/td>\n<\/tr>\n
55<\/td>\nReference ref_14
Reference ref_15
Reference ref_16
Reference ref_17 <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of botulinum type A, B, E and F neurotoxin-producing clostridia<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2013<\/td>\n58<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":340821,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-340814","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/340814","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/340821"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=340814"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=340814"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=340814"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}