{"id":344066,"date":"2024-10-20T00:09:35","date_gmt":"2024-10-20T00:09:35","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-iso-211502015\/"},"modified":"2024-10-25T23:30:15","modified_gmt":"2024-10-25T23:30:15","slug":"bs-en-iso-211502015","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-iso-211502015\/","title":{"rendered":"BS EN ISO 21150:2015"},"content":{"rendered":"

This International Standard gives general guidelines for the detection and identification of the specified microorganism Escherichia coli<\/i> in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations.<\/p>\n

In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis, so as to determine the types of cosmetic products to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621<\/span> <\/span>) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.<\/p>\n

The method described in this International Standard is based on the detection of Escherichia coli<\/i> in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate, depending on the level of detection required.<\/p>\n

\n
\n NOTE\n <\/div>\n

For the detection of Escherichia coli<\/i>, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits).<\/p>\n<\/div>\n

Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other International Standards ( ISO 18415<\/span> <\/span>) may be appropriate. Other methods (e.g. automated) can be substituted for the test presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
4<\/td>\nEuropean foreword <\/td>\n<\/tr>\n
6<\/td>\nForeword <\/td>\n<\/tr>\n
7<\/td>\nIntroduction <\/td>\n<\/tr>\n
9<\/td>\n1\tScope
2\tNormative references
3\tTerms and definitions <\/td>\n<\/tr>\n
10<\/td>\n4\tPrinciple
5\tDiluents and culture media
5.1\tGeneral <\/td>\n<\/tr>\n
11<\/td>\n5.2\tDiluent for the bacterial suspension (tryptone sodium chloride solution)
5.2.1\tGeneral
5.2.2\tComposition
5.2.3\tPreparation
5.3\tCulture media
5.3.1\tGeneral
5.3.2\tAgar medium for the suitability test (see Clause\u00a011) [soybean-casein digest agar medium (SCDA) or tryptic soy agar (TSA)] <\/td>\n<\/tr>\n
12<\/td>\n5.3.3\tEnrichment broth
5.3.4\tSelective agar medium for isolation of Escherichia coli <\/td>\n<\/tr>\n
13<\/td>\n5.3.5\tSelective agar medium for confirmation of Escherichia coli <\/td>\n<\/tr>\n
14<\/td>\n6\tApparatus and glassware
7\tStrains of microorganisms
8\tHandling of cosmetic products and laboratory samples
9\tProcedure
9.1\tGeneral recommendation
9.2\tPreparation of the initial suspension in the enrichment broth
9.2.1\tGeneral <\/td>\n<\/tr>\n
15<\/td>\n9.2.2\tWater-miscible products
9.2.3\tWater-immiscible products
9.2.4\tFilterable products
9.3\tIncubation of the inoculated enrichment broth
9.4\tDetection and identification of Escherichia coli
9.4.1\tIsolation
9.4.2\tIdentification of Escherichia coli <\/td>\n<\/tr>\n
16<\/td>\n10\tExpression of the results (detection of Escherichia coli)
11\tNeutralization of the antimicrobial properties of the product
11.1\tGeneral
11.2\tPreparation of inoculum
11.3\tSuitability of the detection method
11.3.1\tProcedure <\/td>\n<\/tr>\n
17<\/td>\n11.3.2\tInterpretation of suitability test results
12\tTest report <\/td>\n<\/tr>\n
18<\/td>\nAnnex\u00a0A (informative) Other enrichment broths <\/td>\n<\/tr>\n
21<\/td>\nAnnex\u00a0B (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids <\/td>\n<\/tr>\n
22<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

ENISO 21150 Escherichia coli<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2015<\/td>\n26<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":344072,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[125,2641],"product_tag":[],"class_list":{"0":"post-344066","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-40","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/344066","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/344072"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=344066"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=344066"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=344066"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}