This document specifies the minimum requirements for quality control of microbiological culture media and diluents in order to demonstrate their ability to detect microorganisms and to ensure reliability of the microbiological test methods described in the ISO cosmetics microbiology standards. This document describes mainly growth promotion and microbial control tests and is applicable to both commercially ready-to-use culture media and culture media prepared from dehydrated culture media or basic constituents in the user\u2019s laboratory. Other methods can be substituted provided that their equivalence has been demonstrated.<\/p>\n
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| 2<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
| 4<\/td>\n | European foreword Endorsement notice <\/td>\n<\/tr>\n | ||||||
| 7<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
| 8<\/td>\n | Introduction <\/td>\n<\/tr>\n | ||||||
| 9<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions <\/td>\n<\/tr>\n | ||||||
| 11<\/td>\n | 4 Principle 4.1 General information 4.2 pH 4.3 Absence of microbiological growth 4.4 Growth promotion 4.5 Selective and indicative properties <\/td>\n<\/tr>\n | ||||||
| 12<\/td>\n | 5 Diluents, neutralizers and culture media 5.1 General 5.2 Diluents and neutralizers 5.3 Culture media 6 Apparatus and glassware 7 Strains of microorganisms <\/td>\n<\/tr>\n | ||||||
| 14<\/td>\n | 8 Procedure 8.1 General recommendation 8.2 Preparation of strains 8.2.1 General <\/td>\n<\/tr>\n | ||||||
| 17<\/td>\n | 8.2.2 Preparation of bacterial and Candida albicans suspensions 8.2.3 Preparation of Aspergillus brasiliensis spore stock suspension <\/td>\n<\/tr>\n | ||||||
| 18<\/td>\n | 8.2.4 Control of the concentration of the calibrated suspension 8.3 Absence of microbial growth 8.3.1 Solid culture media 8.3.2 Liquid culture media and diluents 8.4 Growth promotion 8.4.1 Solid culture media <\/td>\n<\/tr>\n | ||||||
| 19<\/td>\n | 8.4.2 Liquid culture media 8.5 Selective properties 8.5.1 Solid culture media \u2014 For indicative properties 8.5.2 Solid culture media \u2014 For inhibition properties 9 Expression of results 9.1 Absence of microbial growth 9.1.1 Solid culture media 9.1.2 Liquid culture media 9.2 Growth promotion 9.2.1 Solid culture media 9.2.2 Liquid culture media <\/td>\n<\/tr>\n | ||||||
| 20<\/td>\n | 9.3 Selective and indicative properties 9.3.1 Solid culture media \u2014 For indicative properties 9.3.2 Solid culture media \u2014 For inhibition properties 10 Interpretation and acceptance criteria 10.1 General 10.2 Absence of microbial growth 10.3 Growth promotion 10.3.1 Solid culture media <\/td>\n<\/tr>\n | ||||||
| 21<\/td>\n | 10.3.2 Growth promotion \u2014 Liquid media culture 10.4 Selective properties of solid culture media 10.4.1 For indicative properties 10.4.2 For inhibition properties <\/td>\n<\/tr>\n | ||||||
| 22<\/td>\n | Annex A (normative) Specifications for growth promoting, inhibitory and indicative properties of culture media <\/td>\n<\/tr>\n | ||||||
| 27<\/td>\n | Annex B (informative) Quality control report for culture media <\/td>\n<\/tr>\n | ||||||
| 29<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Cosmetics. Microbiology. Quality control of culture media and diluents used in cosmetics standards<\/b><\/p>\n |