This document specifies a test method and the minimum requirements for the microbicidal activity of a specified disinfection process for the treatment of contaminated textile. This procedure is carried out by using a washing machine as specified in 5.3.2.18 and refers to the disinfection step without prewash. This procedure is not limited to certain types of textile. The suppliers’ instructions are expected to be sufficient if they content the process parameters identified in the test (e.g. dosing disinfectant in whatever washing phase e.g. main wash, rinsing, disinfecting at 40 °C). This document applies to areas and situations where disinfection is medically indicated. Such indications occur in patient care, for example: – in hospitals, in community medical facilities, and in dental institutions; – in clinics of schools, of kindergartens, and of nursing homes; and could occur in the workplace and in the home. It could also include services such as laundries and kitchens supplying products directly for the patients. The method described is intended to determine the activity of a product or product combination under the conditions in which they are used. This is a phase 2, step 2 laboratory test that simulates the conditions of application of the product. EN 14885 specifies in detail the relationship of the various tests to one another and to “use recommendations”.

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65	undefined
72	1 Scope 2 Normative references 3 Terms and definitions
73	4 Requirements
74	5 Test methods 5.1 Principle 5.2 Materials and reagents 5.2.1 Test organisms
75	5.2.2 Culture media and reagents 5.2.2.1 General 5.2.2.2 Water used for preparation of media 5.2.2.3 Hard water for dilution of products for validation tests 5.2.2.4 Water for the test and reference control
76	5.2.2.5 Tryptone Soy Agar (TSA) 5.2.2.6 Tryptone Soy Broth (TSB) 5.2.2.7 Brain Heart Infusion Agar (BHI)
77	5.2.2.8 Malt Extract Agar (MEA) 5.2.2.9 Middlebrook and Cohn 7H10 medium incl. 10 % OADC (7H10) 5.2.2.10 Diluent 5.2.2.11 Neutralizer 5.2.2.12 Sterile defibrinated sheep blood 5.3 Apparatus and glassware 5.3.1 General
78	5.3.2 Usual microbiological laboratory equipment
80	5.4 Preparation of test organism suspensions (test suspension) 5.4.1 General 5.4.2 Preservation and stock cultures of test organisms
81	5.4.3 Working culture and test organisms 5.4.3.1 Bacteria 5.4.3.2 Yeasts and moulds 5.4.3.3 Mycobacteria 5.4.4 Test suspension (N) 5.4.4.1 Bacterial test suspension (N)
82	5.4.4.2 Fungal test suspension (N)
84	5.4.4.3 Mycobacterial test suspension (N)
85	5.4.5 Inoculation of the carriers
86	5.5 Procedure for assessing the microbicidal activity of the product 5.5.1 General 5.5.1.1 Experimental conditions (obligatory and additional) 5.5.1.2 Choice of neutralizer
87	5.5.1.3 Precautions for manipulation of test organisms 5.5.2 Test procedure 5.5.2.1 General 5.5.2.2 Test Na (Determination of microbicidal concentrations) and untreated control N0 (Microbial content on carriers)
88	5.5.2.3 Reference control Nw (Test without detergent or disinfectant) 5.5.2.4 Control RI (Non contaminated cotton carrier in the test procedure and the reference procedure)
89	5.5.2.5 Incubation and counting 5.6 Experimental data and calculation 5.6.1 Explanation of terms and abbreviations 5.6.1.1 Overview of the different suspensions and test mixtures 5.6.1.2 VC-values
90	5.6.2 Calculation 5.6.2.1 General 5.6.2.2 Determination of VC-values
91	5.6.2.3 Calculation of N and N0 5.6.2.4 Calculation of Na
92	5.6.2.5 Calculation of Nw 5.7 Verification of methodology 5.7.1 General
93	5.7.2 Control of weighted mean counts 5.7.3 Basic limits 5.8 Expression of results and precision 5.8.1 Reduction
94	5.8.2 Repetitions 5.9 Interpretation of results – conclusion 5.9.1 General 5.9.2 Microbicidal activity 5.9.2.1 Processes at temperatures < 60 °C to 70 °C
95	5.9.2.2 Processes at temperatures ≥ 60 °C 5.10 Test report
97	Annex A (informative)Referenced strains in national collections
99	Annex B (informative)Suitable neutralizers B.1 General B.2 Neutralizers
100	B.3 Neutralizer added to the agar for counting
101	Annex C (informative)Graphical representations of the test method
102	Annex D (informative)Example of washing machine specification
103	Annex E (informative)Preparation of hard water for using in the test and reference procedures
104	Annex F (informative)Test report (example)
111	Annex G (informative)Example for loading the washing machine

Published By	Publication Date	Number of Pages
BSI	2024	115


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Status	Definitive
Pages	115
Publication Date	2024-01-10
ISBN	978 0 539 30033 8
Standard Number	BS EN 16616:2022 – TC
Title	Tracked Changes. Chemical disinfectants and antiseptics. Chemical-thermal textile disinfection. Test method and requirements (phase 2, step 2)
Descriptors	Testing conditions, Reports, Disinfectants, Suspensions (chemical), Microbiological-resistance tests, Disinfectant tests, Industrial, Bacteriocide-activity determination, Filtration, Bacteriocides, Domestic, Antiseptics, Institutional facilities, Food technology, Test equipment, Microbiological analysis
Publisher	BSI
Committee	CH/216
ICS Codes	11.080.20 - Disinfectants and antiseptics

BS EN 16616:2022 – TC:2024 Edition

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