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BS EN ISO 20418-1:2018

BS EN ISO 20418-1:2018

$102.76

Textiles. Qualitative and quantitative proteomic analysis of some animal hair fibres – Peptide detection using LC-ESI-MS with protein reduction

Published By Publication Date Number of Pages
BSI 2018 20
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This document specifies a qualitative and quantitative testing method to determine the content of wool, cashmere, yak fibres and their blends in textiles by microscope preliminary screening, protein extraction, enzymatic digestion and specific peptides detection using a liquid chromatography-mass spectrometer equipped with electrospray ionization source (LCI-ESI-MC).

This method can be applied to relevant textile products at each process stage (i.e. from raw material to garment) with a homogeneous distribution of the components. It can be applied to different types of textile materials (e.g. staples, tops, yarns and fabrics) that contain wool, cashmere or yak fibres and their blends. The method is based on a preliminary identification of all fibres in the blend on the basis of their morphology, by light microscopy. The proteins are then extracted by a thiourea/urea/dithiothreitol (DTT) solution. An enzymatic digestion by trypsin of the protein extracted from the fibres is carried out. Analysis of the specific markers is performed by LC-MS and the percent composition is calculated.

This method is applicable to samples containing other kinds of fibres than wool, cashmere and yak, by combining its results with the results obtained using the ISO 1833 series and/or the ISO 17751 series.

This document does not apply if fibres of the same animal species are present (e.g. blends of cashmere and mohair); in this case, the quantitative analysis can be performed using microscopic analysis (e.g. ISO 17751 series).

PDF Catalog

PDF Pages PDF Title
2 National foreword
4 European foreword
6 Foreword
7 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Abbreviated terms
5 Reagents
11 6 Apparatus
7 Test method
7.1 Sampling
7.2 Preliminary identification
7.3 Sample preparation
12 7.4 Protein extraction
7.5 Trypsin digestion of the extracted proteins
7.6 Tryptic peptides analysis by LC-ESI-MS
7.7 Calculation and expression of results
13 7.8 Test report
14 Annex A (informative) Parameters
16 Annex B (informative) Markers
17 Annex C (informative) Examples of interlaboratory results
19 Bibliography

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BS EN ISO 20418-1:2018
$102.76