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BS EN ISO 7218:2024

BS EN ISO 7218:2024

$215.11

Microbiology of the food chain. General requirements and guidance for microbiological examinations

Published By Publication Date Number of Pages
BSI 2024 92
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This document specifies general requirements and gives guidance on microbiological examinations. It is applicable to: —     the implementation of specific horizontal or vertical International Standards developed by ISO/TC 34/SC 9 or ISO/TC 34/SC 5 for detection or enumeration of microorganisms, named hereafter “ specific standards”; —     good laboratory practices for microbiology laboratories testing samples from the food chain; —      guidance for microbiological laboratories testing samples from the food chain on the technical requirements for conforming to ISO/IEC 17025. The requirements of this general standard supersede corresponding ones in existing specific standards. Additional instructions for examinations using the polymerase chain reaction (PCR) are specified in ISO 22174. This document is applicable to examinations for bacteria, yeasts and moulds and can be used, if supplemented with specific guidance, for parasites and viruses. It does not apply to examinations for toxins or other metabolites (e.g. amines) from microorganisms. This document is applicable to microbiology of the food chain, from primary production stage to food and animal feed products, including the premises where the food or feed production and handling takes place. It is also applicable to the microbiological examination of water where water is used in food production or is regarded as a food in national legislation.

PDF Catalog

PDF Pages PDF Title
2 undefined
15 4.1 General
4.2 Biosafety considerations
4.3 Laboratory design
4.4 Laboratory areas
4.4.1 General
16 4.4.2 Areas associated with samples and testing
4.4.3 General areas
4.5 Layout and fittings of the premises
4.5.1 Objectives
17 4.5.2 Fittings
4.5.3 Other arrangements for laboratory premises
18 4.5.4 Cleaning and disinfection
5.1 General
5.2 Competence
19 5.3 Verification of ongoing staff competence
5.4 Hygiene
6.1 General
20 6.2 Sterilization and other heating equipment
6.2.1 General
6.2.2 Autoclave
21 6.2.3 Culture media preparator
22 6.2.4 Steamers, including boiling-water baths
6.2.5 Sterilizing oven
23 6.2.6 Microwave oven
24 6.2.7 Hotplate, induction cooker and heating mantle
6.2.8 Gas burner or wire incinerator
25 6.3 Temperature controlled equipment and monitoring devices
6.3.1 General
6.3.2 Incubator
26 6.3.3 Thermostatically controlled bath
27 6.3.4 Heating blocks
28 6.3.5 Refrigerators and cold-storage rooms
29 6.3.6 Freezer and deep freezer/ultra-low temperature freezer
6.3.7 Temperature-monitoring devices, including automatic recorders
30 6.3.8 Balances and gravimetric diluters
31 6.4 Defined volume inoculation equipment
6.4.1 Pipettes and pipettors
32 6.4.2 Dispensers
33 6.4.3 Spiral platers
34 6.4.4 Serial diluters
6.5 Protective cabinets
6.5.1 Description
35 6.5.2 Use
6.5.3 Cleaning and disinfection
36 6.5.4 Maintenance and inspection
6.6 Homogenizers, blenders, mixers and shakers
6.6.1 Homogenizers and blenders
37 6.6.2 Vortex mixers
38 6.7 Stills, deionizers and reverse-osmosis units
6.7.1 Description
6.7.2 Use
6.7.3 Maintenance
6.7.4 Verification
6.8 Separation and concentration equipment
6.8.1 Immunomagnetic separator (IMS)
39 6.8.2 Centrifuge
6.8.3 Filtration systems
6.9 Modified atmosphere equipment
6.9.1 Description
6.9.2 Use
40 6.9.3 Maintenance
6.9.4 Verification
6.10 Other equipment
6.10.1 pH meter
41 6.10.2 Colony-counting device
6.10.3 Timers and timing devices
42 6.10.4 Optical microscope
6.10.5 Glass washers, glassware and other laboratory ware
43 6.10.6 Disposable equipment and consumables
44 6.10.7 Other equipment and software
7.1 Sterilization
7.1.1 General
7.1.2 Sterilization by dry heat
7.1.3 Sterilization by moist heat (steam)
7.2 Decontamination and disinfection
7.2.1 Decontamination of glassware and materials before use
7.2.2 Decontamination of glassware and materials after use
45 7.3 Waste management
7.4 Washing
46 9.1 Sampling techniques and sampling plans
9.1.1 General
9.1.2 Sampling
9.2 Sample transport
47 9.3 Sample receipt
9.4 Sample handling
9.4.1 General
48 9.4.2 Storage before examination
9.4.3 Test portions
9.4.4 Storage of laboratory samples after examination
9.5 Pre-testing of samples
49 10.1 Hygienic precautions during sample preparation and examination
10.1.1 General
10.1.2 Basic precautions
10.1.3 Sample handling
50 10.1.4 Sample handling tools and implements
10.1.5 Spillages
10.1.6 Process controls
10.1.7 Aerosols
51 10.1.8 Molecular methods
10.2 Preparation of initial suspension and dilutions
10.2.1 General
10.2.2 Concentration
11.1 General
52 11.2 Enumeration using a solid medium
11.2.1 General
11.2.2 Pour plate technique
53 11.2.3 Surface plating techniques
54 11.2.4 Enumeration of yeasts and moulds
55 11.2.5 Incubation
11.2.6 Calculation and expression of results obtained with solid culture media
57 11.2.7 Calculations for enumeration methods
64 11.3 Enumeration using liquid media
11.3.1 Principle
11.3.2 General MPN procedure
11.3.3 Limitations of MPN
65 11.3.4 Inoculation procedure
11.3.5 Choice of MPN configuration
66 11.3.6 Incubation
11.3.7 Interpretation and expression of results
11.3.8 Determination of MPN values using MPN calculators
67 11.3.9 Rarity categories
11.4 Estimates of uncertainty of test results
68 12.1 General
12.2 Principle
13.1 General
69 13.2 Preparation of a pure culture
13.3 Confirmation methods
13.3.1 Latex agglutination test
13.3.2 Nucleic acid hybridization or molecular amplification methods
70 13.3.3 Slide agglutination tests
13.4 Identification methods
13.4.1 Biochemical galleries
13.4.2 DNA sequencing
71 13.4.3 Mass spectrometry
14.1 General
72 14.2 Characterization of microorganisms
14.2.1 General
14.2.2 Phenotypic characterization
14.2.3 Molecular characterization
14.3 Selection of control microorganisms
74 16.1 General
75 16.2 Internal quality control
16.2.1 General
16.2.2 Process controls
76 16.2.3 Replicate testing
16.2.4 Spiked samples
16.2.5 IQC assessment using control charts
16.3 External quality assessment
77 17.1 General
17.2 Performance characteristics
17.3 Validation
78 17.4 Verification

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BS EN ISO 7218:2024
$215.11