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BS EN ISO 9167:2019 – TC:2020 Edition

BS EN ISO 9167:2019 – TC:2020 Edition

$217.84

Tracked Changes. Rapeseed and rapeseed meals. Determination of glucosinolates content. Method using high-performance liquid chromatography

Published By Publication Date Number of Pages
BSI 2020 85
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This document specifies a method for the determination of the individual glucosinolates content in rapeseeds and rapeseed meals using high-performance liquid chromatography with gradient elution.

This method was tested on rapeseeds and rapeseed meals (Brassica rapa, Brassica napus and Brassica juncea) but is applicable to other plant materials, on the condition that the occurring glucosinolates previously identified are described in this document. On the contrary, the quantitative analysis of the concerned glucosinolate(s) is not carried out.

NOTE

This method does not determine glucosinolates that are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed and rapeseed meal.

Annex A presents the results of the interlaboratory trials for the gradient elution HPLC method. Annex B presents how to check the titre of the prepared internal standard solution. Annex C presents how to prepare and test the purified sulfatase solution and how to check the desulphation step on the ion exchange column. Annex D presents the HPLC and column performance criteria qualification.

The analysis of glucosinolates content in rapeseed can also be done using an isocratic elution mode. This requires some modifications of the method (internal, standard, HPLC column and HPLC buffers), as described in Annex E.

PDF Catalog

PDF Pages PDF Title
49 National foreword
51 European foreword
53 Foreword
54 Introduction
56 1 Scope
2 Normative references
3 Terms and definitions
57 4 Principle
5 Reagents
5.5 Internal standard.
58 5.6 Sinigrin solution.
5.7 Glucotropaeolin solution.
59 6 Apparatus
6.3 pH-meter.
60 7 Sampling
61 8 Preparation of the test sample
9 Procedure
9.1 Test portion
9.2 Extraction of glucosinolates
62 9.3 Blank test
9.4 Preparation of ion-exchange columns
9.5 Purification and desulfatation
9.6 Chromatography with gradient elution
9.6.1 General
63 9.6.2 Adjustment of the apparatus
64 10 Expression of results
10.1 Calculation of the content of each glucosinolate
10.2 Relative proportionality factors
65 10.3 Calculation of the total glucosinolate content
11 Precision
11.1 Interlaboratory test
11.2 Repeatability
11.3 Reproducibility
12 Test report
67 Annex A (informative) Results of interlaboratory trials — Gradient elution HPLC method
69 Annex B (normative) Checking of the titre of the prepared internal standard solution
70 Annex C (normative) Preparation and test of purified sulfatase solution and checking of the desulphation step on ion-exchange columns
75 Annex D (informative) HPLC system and column performance criteria qualification
77 Annex E (informative) Elution in the isocratic mode
83 Bibliography

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BS EN ISO 9167:2019 - TC
$217.84