BS ISO 20418-2:2018

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Textiles. Qualitative and quantitative proteomic analysis of some animal hair fibres – Peptide detection using MALDI-TOF MS

Published By	Publication Date	Number of Pages
BSI	2018	32

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Categories: 59.060.01 - Textile fibres in general, BSI

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Description

This document specifies a qualitative and quantitative procedure to determine the composition of animal hair fibre blends by MALDI-TOF MS.

The composition of non-animal hair fibres can be measured by methods described in the ISO 1833 series. Both results are then combined to determine the whole composition of fibres.

The method is based on a preliminary identification, by light microscopy, of all fibres in a blend on the basis of their morphology, according to ISO/TR 11827. In case fibres of the same animal species are present (e.g. blends of cashmere and mohair), the method is not applicable and the quantitative analysis can be performed using microscopical analysis (e.g. as described in ISO 17751 series).

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PDF Pages	PDF Title
2	National foreword
6	Foreword
7	Introduction
9	1 Scope 2 Normative references 3 Terms and definitions
10	4 Abbreviated terms 5 Principle 6 Reagents
11	6.12 DTT solution.
13	7 Apparatus
14	8 Test methods 8.1 Sample preparation 8.2 Protein extraction 8.3 Partial purification of extracted proteins using SDS-PAGE 8.4 Trypsin digestion of the extracted proteins
15	8.5 MALDI-TOF MS analysis of the tryptic peptides 8.6 Calculation of the weight percentages
16	8.7 Calibration curves 8.8 Calculation of the content (%) of each animal hair in binary mixtures 8.9 Calculation of the content (%) of each animal hair in ternary mixtures
17	9 Precision 10 Interlaboratory trial 11 Test report
18	Annex A (informative) Example of SDS-PAGE image of extracted proteins
19	Annex B (informative) Animal species-specific peaks in MALDI-TOF MS analysis
20	Annex C (informative) Animal species-specific peaks in MALDI-TOF MS analysis
22	Annex D (informative) Calibration curve for the cashmere-wool blend
23	Annex E (informative) Calibration curve for the cashmere-yak blend
24	Annex F (informative) Repeatability and reproducibility
26	Annex G (informative) Analysis of blind test samples using MALDI-TOF MS method
27	Annex H (informative) Results of interlaboratory trial
31	Bibliography

Additional information

Status	Confirmed
Pages	32
Publication Date	2018-09-06
ISBN	978 0 580 91314 3
Standard Number	BS ISO 20418-2:2018
Title	Textiles. Qualitative and quantitative proteomic analysis of some animal hair fibres – Peptide detection using MALDI-TOF MS
Identical National Standard Of	ISO 20418-2:2018
Descriptors	Mixtures, Chemical tests (textiles), Extraction methods of analysis, Textile fibres, Textile testing, Quantitative analysis, Chemical analysis and testing, Fibre testing (textiles), Regenerated protein fibres, Proteins, Determination of content
Publisher	BSI
Committee	TCI/100
ICS Codes	59.060.01 - Textile fibres in general

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BS ISO 20418-2:2018

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