BS ISO 22196:2011
$142.49
Measurement of antibacterial activity on plastics and other non-porous surfaces
| Published By | Publication Date | Number of Pages |
| BSI | 2011 | 26 |
WARNING — Handling and manipulation of microorganisms which are potentially hazardous requires a high degree of technical competence and may be subject to current national legislation and regulations. Only personnel trained in microbiological techniques should carry out such tests. Appropriate practices for disinfection, sterilization and personal hygiene must be strictly observed.
This International Standard specifies a method of evaluating the antibacterial activity of antibacterial-treated plastics, and other non-porous, surfaces of products (including intermediate products).
It is not intended to be used to evaluate the effects and propagation of bacteria on non-porous surfaces without antibacterial treatments. ISO 846[1] describes tests to evaluate the effects and propagation of bacteria on non-porous surfaces, which are different from those covered by this International Standard (see e.g. ISO 846:1997, method C).
Secondary effects of antibacterial treatments, such as the prevention of biodeterioration and odour, are not covered by this International Standard, which is not intended to be used or referenced as a method to document or claim biodegradability of, for instance, plastics materials. In the case of plastics, biodegradation is covered in ISO 14851[2], ISO 14852[3] and ISO 14855[4] and related standards.
Building materials are excluded, except where they are used in the same manner as treated articles.
Antibacterial-treated textile products are excluded, even if the surfaces are coated or laminated (such products are covered by ISO 20743[5]).
Photocatalytic materials and products are excluded (such materials and products are covered by ISO 27447[6]).
The results obtained should include a reference to this International Standard and the conditions used. Results obtained with this International Standard indicate antibacterial activity under the specified experimental conditions used, and do not reflect activity under other circumstances where a variety of factors, such as temperature, humidity, different bacterial species, nutrient conditions, etc., have to be considered. A minimum diffusion of the antibacterial agents/chemicals into the test inoculum is necessary with this procedure.
It is recommended that workers consult ISO 7218.
PDF Catalog
| PDF Pages | PDF Title |
|---|---|
| 9 | Scope Normative references |
| 10 | Terms and definitions Materials Bacteria to be used for the tests |
| 11 | Reagents, culture media and solutions Nonionic surfactant Biological materials Culture medium General Suspension medium — 1/500 nutrient broth (1/500 NB) Nutrient agar |
| 12 | Plate count agar Slant culture medium Soybean casein digest broth with lecithin and polyoxyethylen Phosphate buffer solution Phosphate-buffered physiological saline Apparatus |
| 13 | Sterilization of apparatus and storage of stock cultures Dry-heat sterilization High-pressure steam sterilization Preparation of glassware Maintenance of stock cultures |
| 14 | Procedure Pre-culture of bacteria Preparation of test specimens Preparation of test inoculum Inoculation of test specimens |
| 16 | Incubation of the inoculated test specimens Recovery of bacteria from test specimens Test specimens immediately after inoculation Test specimens after incubation Determining the viable bacteria count by the pour plate cult |
| 17 | Expression of results Determination of the number of viable bacteria Conditions for a valid test Calculation of the antibacterial activity |
| 18 | Effectiveness of the antibacterial agent Repeatability and reproducibility Test report |
