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BSI PD ISO/TS 22292:2021

BSI PD ISO/TS 22292:2021

$198.66

Nanotechnologies. 3D image reconstruction of rod-supported nano-objects using transmission electron microscopy

Published By Publication Date Number of Pages
BSI 2021 54
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This document provides guidance for sample preparation, data acquisition by transmission electron microscopy, data processing, and three-dimensional image reconstruction to measure size and shape parameters of nano-objects on rod-shaped supports. The method is applicable to samples dispersed on or within an electron-transparent rod-shaped support.

PDF Catalog

PDF Pages PDF Title
2 National foreword
7 Foreword
8 Introduction
11 1 Scope
2 Normative references
3 Terms and definitions
12 3.1 Nanotechnology-related terms
3.2 Instrument-related terms
13 3.3 Measurement-related terms
14 4 Sample considerations
4.1 General
4.2 Choice of sample rod diameter
5 Instrument factors
5.1 Microscope set up
5.1.1 General
15 5.1.2 Acceleration voltage
5.1.3 Convergence semi-angle
5.1.4 Collection angle
5.1.5 Microscope magnification
5.1.6 Number of pixels of the detector
16 5.1.7 Image acquisition time
5.2 Microscope calibration
17 6 Image capture (data acquisition)
6.1 General
6.2 Procedure
20 7 Data alignment and volume reconstruction
7.1 General
7.2 Procedure
8 Reconstructed volume evaluation and data analysis
8.1 General
8.2 Identification of nanoparticles and 3D volume
21 8.3 Thresholding for measurand extraction
23 9 Expression of results
9.1 Extracting parameters for each well-separated nano-object
24 9.2 Measurement uncertainty
9.3 Sources of errors
9.3.1 Error arising from sample that is not representative of the object of interest
9.3.2 Acquisition of 2D projected images
26 9.3.3 Instrument calibration
9.3.4 Alignment of the projected images
9.3.5 Reconstruction of the 3D volume
9.3.6 Discrete representation of the objects (nanoparticles) in 3D
27 9.3.7 Interpretation of the obtained measurands
9.3.8 Limited number of observed objects (nanoparticles)
28 10 Test report
30 Annex A (informative) Sample preparation
37 Annex B (informative) STEM set up
39 Annex C (normative) Tomography reconstruction and visualization software packages
41 Annex D (informative) Microscope data collection parameters
42 Annex E (informative) Case study: Metal nanoparticle, ILC results
47 Annex F (informative) Case study — Organic nanoparticles — Sample preparation and use
48 Annex G (informative) Particle distortions arising from FBP and SIRT reconstruction methods
50 Annex H (informative) Uncertainty budget
52 Bibliography

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BSI PD ISO/TS 22292:2021
$198.66