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BSI PD ISO/TS 23366:2023

BSI PD ISO/TS 23366:2023

$142.49

Nanotechnologies. Performance evaluation requirements for quantifying biomolecules using fluorescent nanoparticles in immunohistochemistry

Published By Publication Date Number of Pages
BSI 2023 28
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PDF Catalog

PDF Pages PDF Title
2 undefined
7 Foreword
8 Introduction
9 1 Scope
2 Normative references
3 Terms and definitions
10 4 Principle
11 5 Selection of fluorescent nanoparticles
5.1 General
5.2 Characteristics of nanoparticle
12 6 Quantification system
6.1 Overall design
13 6.2 Antibody
6.2.1 General
6.2.2 Antibody type
6.2.3 Target antigen
14 6.2.4 Applicability of antibody to method
6.2.5 Animal species
6.2.6 Immunoglobulin class
6.2.7 Titre
15 6.2.8 Specificity
6.3 Staining procedure
6.3.1 Staining conditions
6.3.2 Robustness of staining procedure
16 6.4 Image processing
6.4.1 Image quality and relevant factors
6.4.2 Selection of image processing software
17 7 Comparability of results
7.1 Light source adjustment
7.2 Reference material
8 Performance characteristics
8.1 Background
8.1.1 General
18 8.1.2 Autofluorescence
8.1.3 Nonspecific absorption of nanoparticles
8.1.4 Nonspecific absorption of antibodies
8.1.5 Loss of intensity and interferences
19 8.2 Reference material dependent indices
8.2.1 General
8.2.2 Limit of detection (LOD)
8.2.3 Limit of quantification (LOQ)
8.2.4 Linearity and dynamic range
20 8.3 Robustness
9 Validation and verification
9.1 General
9.2 Single lab precision
21 9.3 Reproducibility
10 Reporting
22 Annex A (informative) Example of the reference material
24 Annex B (informative) Example of the nanoparticle aggregation/agglomeration evaluation method
26 Bibliography

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BSI PD ISO/TS 23366:2023
$142.49