{"id":291335,"date":"2024-10-19T19:46:55","date_gmt":"2024-10-19T19:46:55","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-217032019\/"},"modified":"2024-10-25T16:48:46","modified_gmt":"2024-10-25T16:48:46","slug":"bs-iso-217032019","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-217032019\/","title":{"rendered":"BS ISO 21703:2019"},"content":{"rendered":"

This document provides microbiological test methods for enumeration and detection of aerobic mesophilic bacteria, detection of Escherichia coli<\/i> and Pseudomonas aeruginosa<\/i> in liquid hand dishwashing.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nNational foreword <\/td>\n<\/tr>\n
7<\/td>\nForeword <\/td>\n<\/tr>\n
9<\/td>\n1 Scope
2 Normative references
3 Terms and definitions <\/td>\n<\/tr>\n
10<\/td>\n4 Principle
5 Diluents and culture media
5.1 General
5.2 Neutralizing diluent and diluents
5.2.1 General <\/td>\n<\/tr>\n
11<\/td>\n5.2.2 Neutralizing diluent: Fluid casein digest \u2014 soy lecithin \u2014 polysorbate 20 medium (SCDLP 20 broth)
5.2.3 Diluent for the bacterial suspension (Tryptone sodium chloride solution)
5.3 Culture medium for counting
5.3.1 General
5.3.2 Soybean-casein digest agar medium (SCDA) or tryptic soy agar (TSA) <\/td>\n<\/tr>\n
12<\/td>\n5.3.3 Other medium for counting
5.4 Culture medium for detection
5.4.1 General
5.4.2 Enrichment broth: Eugon LT 100 broth <\/td>\n<\/tr>\n
13<\/td>\n5.5 Culture media for isolation and identification
5.5.1 MacConkey agar medium <\/td>\n<\/tr>\n
14<\/td>\n5.5.2 Levine eosin-methylene blue agar medium (EMB agar medium)
5.5.3 Cetrimide agar medium <\/td>\n<\/tr>\n
15<\/td>\n5.5.4 Pseudomonas agar medium for detection of pyocyanin (Pseudomonas agar P)
6 Apparatus and glassware
7 Strains of microorganisms
8 Handling of the product and laboratory samples <\/td>\n<\/tr>\n
16<\/td>\n9 Procedure
9.1 General recommendation
9.2 Preparation of the initial suspension
9.3 Enumeration methods
9.3.1 Dilutions for enumeration
9.3.2 Plate-count methods <\/td>\n<\/tr>\n
18<\/td>\n10 Method for detection and identification
10.1 Enrichment
10.2 Isolation <\/td>\n<\/tr>\n
19<\/td>\n10.3 Detection of aerobic mesophilic bacteria
10.4 Detection and Identification of Escherichia coli
10.4.1 General
10.4.2 Gram\u2019s stain
10.4.3 Culture on Levine eosin-methylene blue agar medium (EMB agar medium)
10.4.4 Expression of results
10.5 Detection and identification of Pseudomonas aeruginosa
10.5.1 General <\/td>\n<\/tr>\n
20<\/td>\n10.5.2 Gram\u2019s stain
10.5.3 Oxidase test
10.5.4 Culture on Pseudomonas agar medium for detection of pyocyanin
10.5.5 Expression of results
11 Neutralization of the antimicrobial properties of the product
11.1 General
11.2 Preparation of inoculum
11.3 Suitability of counting methods
11.3.1 Principle <\/td>\n<\/tr>\n
21<\/td>\n11.3.2 Suitability test of the pour-plate method
11.3.3 Suitability of the surface spread method
11.3.4 Suitability of the membrane filtration method
11.4 Suitability of the detection method
11.4.1 Procedure <\/td>\n<\/tr>\n
22<\/td>\n11.4.2 Interpretation
12 Test report <\/td>\n<\/tr>\n
23<\/td>\nAnnex A (informative) Neutralizers of antimicrobial activity of preservatives and rinsing liquids <\/td>\n<\/tr>\n
24<\/td>\nAnnex B (informative) Other culture media <\/td>\n<\/tr>\n
26<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Surface active agents. Microbiology. Microbiological test methods for liquid hand dishwashing<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2019<\/td>\n28<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":291341,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[1030,2641],"product_tag":[],"class_list":{"0":"post-291335","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-71-100-40","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/291335","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/291341"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=291335"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=291335"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=291335"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}