{"id":302974,"date":"2024-10-19T20:42:19","date_gmt":"2024-10-19T20:42:19","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-pd-iso-ts-220822020\/"},"modified":"2024-10-25T18:14:15","modified_gmt":"2024-10-25T18:14:15","slug":"bsi-pd-iso-ts-220822020","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-pd-iso-ts-220822020\/","title":{"rendered":"BSI PD ISO\/TS 22082:2020"},"content":{"rendered":"
This document specifies a method for rapidly assessing nanomaterial toxicity (fish early life stage, 0 HPF to 120 HPF). It includes information on the importance of acellular chorion removal, detailed chorion removal procedures, and a complete protocol for the toxicity assessment of nanomaterials using dechorionated zebrafish embryos. The focus of this document is on testing nanomaterial toxicity.<\/p>\n
PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
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2<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
6<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
7<\/td>\n | Introduction <\/td>\n<\/tr>\n | ||||||
8<\/td>\n | 1 Scope 2 Normative references 3 Terms and definitions <\/td>\n<\/tr>\n | ||||||
9<\/td>\n | 4 Abbreviated terms 5 Materials <\/td>\n<\/tr>\n | ||||||
10<\/td>\n | 6 Apparatus 6.1 Technical equipment 6.2 Analytical instruments <\/td>\n<\/tr>\n | ||||||
11<\/td>\n | 7 Procedures 7.1 Culture 7.1.1 Zebrafish strain 7.1.2 Feeding regime 7.1.3 Photoperiod 7.1.4 Temperature and pH 7.1.5 Tank material 7.2 Spawning stimulation <\/td>\n<\/tr>\n | ||||||
12<\/td>\n | 7.3 Dechorionation of embryos 7.3.1 Methods 7.3.2 Enzymatic dechorionation method <\/td>\n<\/tr>\n | ||||||
13<\/td>\n | 7.4 Preparation of nanomaterials stock solution 7.5 Testing concentrations 7.6 Dispersion of nanomaterials 7.7 Positive chemical: 3,4-dichloroaniline <\/td>\n<\/tr>\n | ||||||
14<\/td>\n | 7.8 Test methods 7.8.1 Water quality measurement 7.8.2 Preparation of dechorionated embryos 7.8.3 Exposure 7.8.4 Endpoints observation <\/td>\n<\/tr>\n | ||||||
15<\/td>\n | 7.9 Data analysis 8 Test report 8.1 Test procedure 8.2 Information to be included in the report 8.2.1 Test nanomaterials 8.2.2 Test species 8.2.3 Materials and apparatus <\/td>\n<\/tr>\n | ||||||
16<\/td>\n | 8.2.4 Maintenance 8.2.5 Test design 8.2.6 Test conditions 8.2.7 Results <\/td>\n<\/tr>\n | ||||||
17<\/td>\n | Annex A (informative) Mechanical dechorionation method <\/td>\n<\/tr>\n | ||||||
18<\/td>\n | Annex B (informative) Zebrafish embryo spawning procedures <\/td>\n<\/tr>\n | ||||||
19<\/td>\n | Annex C (informative) Results validation <\/td>\n<\/tr>\n | ||||||
20<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Nanotechnologies. Assessment of nanomaterial toxicity using dechorionated zebrafish embryo<\/b><\/p>\n |