{"id":335917,"date":"2024-10-19T23:28:11","date_gmt":"2024-10-19T23:28:11","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bsi-20-30396933-dc-2020\/"},"modified":"2024-10-25T22:25:13","modified_gmt":"2024-10-25T22:25:13","slug":"bsi-20-30396933-dc-2020","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bsi-20-30396933-dc-2020\/","title":{"rendered":"BSI 20\/30396933 DC 2020"},"content":{"rendered":"<p>This document specifies a method for the determination of the content of the total vitamin A (retinol), vitamin E (\u03b1-tocopherol) and vitamin D<sub>3<\/sub> (cholecalciferol) content in animal feed using solid phase extraction (SPE) clean-up and high-performance liquid chromatography (HPLC).<\/p>\n<div class=\"non-normative-note\">\n<div class=\"note-label\">\n  NOTE&nbsp;1\n <\/div>\n<p>The procedure enables also determination of vitamin D<sub>2<\/sub> but with the use of another internal standard. The method is fully validated only for vitamin D<sub>3<\/sub>.<\/p>\n<\/div>\n<p>The method has been successfully tested in collaborative trial for complete feed for broilers, pigs, and turkey, for premixture for broilers and piglets, for complementary feed for cows and mineral feed within the following ranges:<\/p>\n<ul>\n<li>\n<p>vitamin&nbsp;A: 4&nbsp;365&nbsp;IU\/kg \u2013 4&nbsp;118&nbsp;352&nbsp;IU\/kg;<\/p>\n<\/li>\n<li>\n<p>vitamin&nbsp;E: 22&nbsp;mg\/kg \u2013 13&nbsp;800&nbsp;mg\/kg<\/p>\n<\/li>\n<li>\n<p>vitamin&nbsp;D<sub>3<\/sub>: 1&nbsp;668&nbsp;IU\/kg \u2013 1&nbsp;638&nbsp;150&nbsp;IU\/kg.<\/p>\n<\/li>\n<\/ul>\n<p>Quantification limits of 1&nbsp;100&nbsp;IU for vitamin A\/kg (using UV-detection), 4&nbsp;mg for vitamin E\/kg (using UV-detection), 2&nbsp;mg for vitamin E\/kg (using fluorescence detection) and 2&nbsp;000&nbsp;IU for vitamin D\/kg (using UV-detection) should be normally achieved.<\/p>\n<div class=\"non-normative-note\">\n<div class=\"note-label\">\n  NOTE&nbsp;2\n <\/div>\n<p>The limits of quantification are minimum limits which were not determined within the validation study. Lower limits of quantification are possible but is validated by the user.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p><b>BS EN 17547. Animal feeding stuffs: Methods of sampling and analysis. Determination of vitamin A, E and D content. Method using solid phase extraction clean-up and High Performance Liquid Chromatography<\/b><\/p>\n<table class=\"shortdes-table\" style=\"width: 100%\" border=\"0\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td>Published By<\/td>\n<td>Publication Date<\/td>\n<td>Number of Pages<\/td>\n<\/tr>\n<tr>\n<td><a href=\"https:\/\/pdfstandards.shop\/product-category\/publishers\/bsi\/\"><b>BSI<\/b><\/a><\/td>\n<td>2020<\/td>\n<td>41<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":335920,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[2641],"product_tag":[],"class_list":{"0":"post-335917","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-bsi","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/335917","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/335920"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=335917"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=335917"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=335917"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}