This document specifies a horizontal method for<\/p>\n
the detection of L. monocytogenes<\/i>, and<\/p>\n<\/li>\n the detection of Listeria<\/i> spp. (including L. monocytogenes<\/i>).<\/p>\n<\/li>\n<\/ul>\n This document is applicable to<\/p>\n products intended for human consumption and for the feeding of animals, and<\/p>\n<\/li>\n environmental samples in the area of food production and food handling.<\/p>\n<\/li>\n<\/ul>\n It is possible that certain additionally described Listeria<\/i> species may not be detected or confirmed by this method.[<\/sup>5<\/sup>],<\/sup>[10]<\/sup>,<\/sup>[12]<\/sup>,[<\/sup>14<\/sup>],[<\/sup>25<\/sup>],[<\/sup>26<\/sup>],[<\/sup>27<\/sup>]<\/sup><\/p>\n Microbiology of the food chain. Horizontal method for the detection and enumeration of Listeria<\/i> monocytogenes<\/i> and of Listeria<\/i> spp. – Detection method<\/b><\/p>\n\n
PDF Catalog<\/h4>\n
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\n PDF Pages<\/th>\n PDF Title<\/th>\n<\/tr>\n \n 2<\/td>\n National foreword <\/td>\n<\/tr>\n \n 4<\/td>\n European foreword <\/td>\n<\/tr>\n \n 9<\/td>\n Foreword <\/td>\n<\/tr>\n \n 10<\/td>\n Introduction <\/td>\n<\/tr>\n \n 11<\/td>\n 1 Scope
2 Normative references <\/td>\n<\/tr>\n\n 12<\/td>\n 3 Terms and definitions
4 Principle
4.1 General
4.2 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half-Fraser broth) <\/td>\n<\/tr>\n\n 13<\/td>\n 4.3 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
4.4 Plating out and identification
4.5 Confirmation
5 Culture media and reagents
6 Equipment and consumables <\/td>\n<\/tr>\n\n 14<\/td>\n 7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Test portion and initial suspension
9.2 Primary enrichment <\/td>\n<\/tr>\n\n 15<\/td>\n 9.3 Secondary enrichment
9.4 Plating out and identification
9.4.1 General
9.4.2 Agar Listeria according to Ottaviani and Agosti <\/td>\n<\/tr>\n\n 16<\/td>\n 9.4.3 Second selective medium
9.5 Confirmation of Listeria monocytogenes or Listeria\u00a0spp.
9.5.1 Selection of colonies for confirmation
9.5.2 Confirmation of L.\u00a0monocytogenes <\/td>\n<\/tr>\n\n 20<\/td>\n 9.5.3 Confirmation of Listeria\u00a0spp. <\/td>\n<\/tr>\n \n 21<\/td>\n 9.6 Interpretation of morphological and physiological properties and of the biochemical reactions
9.7 Additional characterization of isolated strains (optional)
10 Expression of results
11 Performance characteristics of the method
11.1 Method validation studies
11.2 Sensitivity
11.3 Specificity
11.4 Level of detection (LOD50) <\/td>\n<\/tr>\n\n 22<\/td>\n 12 Test report
13 Quality assurance <\/td>\n<\/tr>\n\n 23<\/td>\n Annex\u00a0A (normative) Diagram of procedure <\/td>\n<\/tr>\n \n 24<\/td>\n Annex\u00a0B (normative) Composition and preparation of culture media and reagents <\/td>\n<\/tr>\n \n 37<\/td>\n Annex\u00a0C (informative) Distinction of Listeria\u00a0spp. from other genera <\/td>\n<\/tr>\n \n 38<\/td>\n Annex\u00a0D (informative) Reactions for the identification of Listeria species <\/td>\n<\/tr>\n \n 40<\/td>\n Annex\u00a0E (informative) Listeria selective agars <\/td>\n<\/tr>\n \n 41<\/td>\n Annex\u00a0F (informative) Results of the interlaboratory studies for detection of Listeria monocytogenes <\/td>\n<\/tr>\n \n 45<\/td>\n Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" \n\n
\n Published By<\/td>\n Publication Date<\/td>\n Number of Pages<\/td>\n<\/tr>\n \n BSI<\/b><\/a><\/td>\n 2017<\/td>\n 50<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":340789,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-340785","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/340785","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/340789"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=340785"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=340785"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=340785"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}