{"id":386844,"date":"2024-10-20T03:37:20","date_gmt":"2024-10-20T03:37:20","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-iso-134932021-tc\/"},"modified":"2024-10-26T06:37:09","modified_gmt":"2024-10-26T06:37:09","slug":"bs-iso-134932021-tc","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-iso-134932021-tc\/","title":{"rendered":"BS ISO 13493:2021 – TC"},"content":{"rendered":"

This document specifies the liquid chromatographic (LC) method for the determination of chloramphenicol content of muscle tissue of meat, including livestock and poultry.<\/p>\n

This document specifies the liquid chromatography tandem mass spectrometry method (LC-MS\/MS) for the determination of chloramphenicol content of muscle tissue, casing, liver of meat and meat products, including livestock and poultry.<\/p>\n

This document specifies LC-MS\/MS as the reference method.<\/p>\n

The LC method is suitable for the determination of chloramphenicol content greater than 6,5 mg\/kg.<\/p>\n

LC-MS\/MS is suitable for the determination of chloramphenicol content greater than 0,1 ?g\/kg.<\/p>\n

Test samples which have deteriorated cannot be analysed with this method.<\/p>\n

PDF Catalog<\/h4>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
1<\/td>\n30447121 <\/td>\n<\/tr>\n
31<\/td>\nA-30401653 <\/td>\n<\/tr>\n
32<\/td>\nNational foreword <\/td>\n<\/tr>\n
36<\/td>\nForeword <\/td>\n<\/tr>\n
37<\/td>\n1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Liquid chromatographic method <\/td>\n<\/tr>\n
38<\/td>\n4.2 Liquid chromatography tandem mass spectrometry method
5 Sampling
6 Preparation of test sample
7 Test method of liquid chromatography
7.1 Reagents
7.1.2 Nitrogen, suitable for evaporating solvents.
7.1.6 Acetonitrile, HPLC grade. <\/td>\n<\/tr>\n
39<\/td>\n7.2 Apparatus <\/td>\n<\/tr>\n
40<\/td>\n7.3 Procedure
7.3.1 General
7.3.2 Test portion
7.3.3 Preparation of extract
7.3.4 Solid-phase extraction
7.3.5 Liquid-liquid extraction <\/td>\n<\/tr>\n
41<\/td>\n7.3.6 Chromatographic analysis
7.4 Calculation <\/td>\n<\/tr>\n
42<\/td>\n7.5 Precision
7.5.1 Interlaboratory test
7.5.2 Repeatability
7.5.3 Reproducibility <\/td>\n<\/tr>\n
43<\/td>\n8 Test method of liquid chromatography tandem mass spectrometry method (reference method)
8.1 Reagents and materials
8.1.8 Methanol and water solution I (volume ratio 2:8).
8.1.9 Methanol and water solution II (volume ratio 1:1). <\/td>\n<\/tr>\n
44<\/td>\n8.1.17 Acetonitrile and water solution (volume ratio 1:9).
8.2 Apparatus
8.2.1 LC-MS\/MS equipment.
8.3 Procedure
8.3.1 General
8.3.2 Test portion <\/td>\n<\/tr>\n
45<\/td>\n8.3.3 Preparation of extract
8.3.4 Solid-phase extraction
8.3.5 LC-MS\/MS analysis <\/td>\n<\/tr>\n
47<\/td>\n8.4 Calculation
8.5 Precision
8.5.1 Interlaboratory test
8.5.2 Repeatability <\/td>\n<\/tr>\n
48<\/td>\n8.5.3 Reproducibility
9 Test report <\/td>\n<\/tr>\n
49<\/td>\nAnnex A (informative) HPLC chromatogram of chloramphenicol standard solution and LC-MS\/MS chromatogram of chloramphenicol standard solution <\/td>\n<\/tr>\n
52<\/td>\nBibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Tracked Changes. Meat and meat products. Determination of chloramphenicol content. Reference method<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2021<\/td>\n54<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":386852,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[967,2641],"product_tag":[],"class_list":{"0":"post-386844","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-67-120-10","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/386844","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/386852"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=386844"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=386844"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=386844"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}