This document specifies the requirements for the performance testing of membrane filters used for the retention followed by direct enumeration of microorganisms by culture methods. This document is applicable to membrane filters which are used for retention followed by direct enumeration of specific microorganisms on solid media or on other devices containing media, like absorbent pads[19]. This document is not applicable for membrane filters used for concentration and elution or for qualitative methods. These tests are applicable to the membrane filters intended for the microbiological analysis of different types of water, such as: \u2014 drinking water, bottled water and other types of water with expected low numbers of microorganisms; \u2014 water with expected higher numbers of microorganisms, for example, surface water and process water. These tests are intended to demonstrate the suitability of the whole system (membrane filter together with the culture medium including the filtration step) required for the specific tests described in References [3], [6], [8], [10], [12] and [13]. This document applies to: \u2014 manufacturers producing membrane filters; \u2014 microbiological laboratories using membrane filters for their own testing or providing these to other end users.<\/p>\n
| PDF Pages<\/th>\n | PDF Title<\/th>\n<\/tr>\n | ||||||
|---|---|---|---|---|---|---|---|
| 2<\/td>\n | undefined <\/td>\n<\/tr>\n | ||||||
| 4<\/td>\n | European foreword Endorsement notice <\/td>\n<\/tr>\n | ||||||
| 7<\/td>\n | Foreword <\/td>\n<\/tr>\n | ||||||
| 9<\/td>\n | Introduction <\/td>\n<\/tr>\n | ||||||
| 11<\/td>\n | 1 \u200bScope 2 \u200bNormative references 3 \u200bTerms and definitions <\/td>\n<\/tr>\n | ||||||
| 12<\/td>\n | 3.1 \u200bGeneral terminology <\/td>\n<\/tr>\n | ||||||
| 13<\/td>\n | 3.2 \u200bTerminology of performance testing <\/td>\n<\/tr>\n | ||||||
| 14<\/td>\n | 3.3 \u200bTerminology for test microorganisms <\/td>\n<\/tr>\n | ||||||
| 15<\/td>\n | 4 \u200bPrinciple 4.1 \u200bGeneral 4.1.1 \u200bIntroduction <\/td>\n<\/tr>\n | ||||||
| 16<\/td>\n | 4.1.2 \u200bBatch testing 4.1.3 \u200bSupplementary testing <\/td>\n<\/tr>\n | ||||||
| 17<\/td>\n | 4.2 \u200bPerformance testing 4.2.1 \u200bModules for batch and supplementary testing 4.2.2 \u200bAbsence of microbial contamination <\/td>\n<\/tr>\n | ||||||
| 18<\/td>\n | 5 \u200bApparatus and glassware 6 \u200bCulture media and diluents 7 \u200bPreparation of microorganisms for performance testing 7.1 \u200bGeneral 7.2 \u200bReference count 7.2.1 \u200bQuantitative productivity testing <\/td>\n<\/tr>\n | ||||||
| 19<\/td>\n | 7.2.2 \u200bQualitative selectivity testing 7.2.3 \u200bQualitative specificity testing 7.3 \u200bPreparation of a standardized test suspension using a working culture 7.3.1 \u200bGeneral 7.3.2 \u200bPreparation of the working culture <\/td>\n<\/tr>\n | ||||||
| 20<\/td>\n | 7.3.3 \u200bPreparation of a standardized test suspension (inoculum) for the test 7.4 \u200bPreparation of a test suspension using reference material 8 \u200bSampling of membrane filters for testing <\/td>\n<\/tr>\n | ||||||
| 21<\/td>\n | 9 \u200bProcedure 9.1 \u200bGeneral 9.2 \u200bInoculation by spread plate technique 9.2.1 \u200bGeneral <\/td>\n<\/tr>\n | ||||||
| 22<\/td>\n | 9.2.2 \u200bInoculation 9.3 \u200bInoculation by membrane filtration technique 9.3.1 \u200bGeneral 9.3.2 \u200bInoculation <\/td>\n<\/tr>\n | ||||||
| 23<\/td>\n | 9.4 \u200bIncubation and counting 9.5 \u200bTest for absence of microbial contamination 10 \u200bCalculation, expression and interpretation of results 10.1 \u200bGeneral 10.2 \u200bProductivity testing <\/td>\n<\/tr>\n | ||||||
| 24<\/td>\n | 10.3 \u200bSelectivity testing 10.4 \u200bSpecificity testing 11 \u200bDocumentation of test results 11.1 \u200bTest report <\/td>\n<\/tr>\n | ||||||
| 25<\/td>\n | 11.2 \u200bInformation provided by the manufacturer 11.3 \u200bTraceability <\/td>\n<\/tr>\n | ||||||
| 26<\/td>\n | Annex A (normative) Diagram of the procedure for batch testing <\/td>\n<\/tr>\n | ||||||
| 27<\/td>\n | Annex B (informative) Example of a card for recording test results from batch testing <\/td>\n<\/tr>\n | ||||||
| 28<\/td>\n | Annex C (informative) Quantitative supplementary testing of membrane filters <\/td>\n<\/tr>\n | ||||||
| 31<\/td>\n | Annex D (informative) Qualitative supplementary testing of membrane filters <\/td>\n<\/tr>\n | ||||||
| 35<\/td>\n | Annex E (informative) Practical example of quantitative batch testing and quantitative supplementary testing by the end user <\/td>\n<\/tr>\n | ||||||
| 45<\/td>\n | Bibliography <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":" Water quality. Requirements for the performance testing of membrane filters used for direct enumeration of microorganisms by culture methods<\/b><\/p>\n |