{"id":464830,"date":"2024-10-20T10:37:10","date_gmt":"2024-10-20T10:37:10","guid":{"rendered":"https:\/\/pdfstandards.shop\/product\/uncategorized\/bs-en-iso-72182024\/"},"modified":"2024-10-26T19:34:53","modified_gmt":"2024-10-26T19:34:53","slug":"bs-en-iso-72182024","status":"publish","type":"product","link":"https:\/\/pdfstandards.shop\/product\/publishers\/bsi\/bs-en-iso-72182024\/","title":{"rendered":"BS EN ISO 7218:2024"},"content":{"rendered":"

This document specifies general requirements and gives guidance on microbiological examinations. It is applicable to: \u2014     the implementation of specific horizontal or vertical International Standards developed by ISO\/TC 34\/SC 9 or ISO\/TC 34\/SC 5 for detection or enumeration of microorganisms, named hereafter \u201c specific standards\u201d; \u2014     good laboratory practices for microbiology laboratories testing samples from the food chain; \u2014      guidance for microbiological laboratories testing samples from the food chain on the technical requirements for conforming to ISO\/IEC 17025. The requirements of this general standard supersede corresponding ones in existing specific standards. Additional instructions for examinations using the polymerase chain reaction (PCR) are specified in ISO 22174. This document is applicable to examinations for bacteria, yeasts and moulds and can be used, if supplemented with specific guidance, for parasites and viruses. It does not apply to examinations for toxins or other metabolites (e.g. amines) from microorganisms. This document is applicable to microbiology of the food chain, from primary production stage to food and animal feed products, including the premises where the food or feed production and handling takes place. It is also applicable to the microbiological examination of water where water is used in food production or is regarded as a food in national legislation.<\/p>\n

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PDF Pages<\/th>\nPDF Title<\/th>\n<\/tr>\n
2<\/td>\nundefined <\/td>\n<\/tr>\n
15<\/td>\n4.1 General
4.2 Biosafety considerations
4.3 Laboratory design
4.4 Laboratory areas
4.4.1 General <\/td>\n<\/tr>\n
16<\/td>\n4.4.2 Areas associated with samples and testing
4.4.3 General areas
4.5 Layout and fittings of the premises
4.5.1 Objectives <\/td>\n<\/tr>\n
17<\/td>\n4.5.2 Fittings
4.5.3 Other arrangements for laboratory premises <\/td>\n<\/tr>\n
18<\/td>\n4.5.4 Cleaning and disinfection
5.1 General
5.2 Competence <\/td>\n<\/tr>\n
19<\/td>\n5.3 Verification of ongoing staff competence
5.4 Hygiene
6.1 General <\/td>\n<\/tr>\n
20<\/td>\n6.2 Sterilization and other heating equipment
6.2.1 General
6.2.2 Autoclave <\/td>\n<\/tr>\n
21<\/td>\n6.2.3 Culture media preparator <\/td>\n<\/tr>\n
22<\/td>\n6.2.4 Steamers, including boiling-water baths
6.2.5 Sterilizing oven <\/td>\n<\/tr>\n
23<\/td>\n6.2.6 Microwave oven <\/td>\n<\/tr>\n
24<\/td>\n6.2.7 Hotplate, induction cooker and heating mantle
6.2.8 Gas burner or wire incinerator <\/td>\n<\/tr>\n
25<\/td>\n6.3 Temperature controlled equipment and monitoring devices
6.3.1 General
6.3.2 Incubator <\/td>\n<\/tr>\n
26<\/td>\n6.3.3 Thermostatically controlled bath <\/td>\n<\/tr>\n
27<\/td>\n6.3.4 Heating blocks <\/td>\n<\/tr>\n
28<\/td>\n6.3.5 Refrigerators and cold-storage rooms <\/td>\n<\/tr>\n
29<\/td>\n6.3.6 Freezer and deep freezer\/ultra-low temperature freezer
6.3.7 Temperature-monitoring devices, including automatic recorders <\/td>\n<\/tr>\n
30<\/td>\n6.3.8 Balances and gravimetric diluters <\/td>\n<\/tr>\n
31<\/td>\n6.4 Defined volume inoculation equipment
6.4.1 Pipettes and pipettors <\/td>\n<\/tr>\n
32<\/td>\n6.4.2 Dispensers <\/td>\n<\/tr>\n
33<\/td>\n6.4.3 Spiral platers <\/td>\n<\/tr>\n
34<\/td>\n6.4.4 Serial diluters
6.5 Protective cabinets
6.5.1 Description <\/td>\n<\/tr>\n
35<\/td>\n6.5.2 Use
6.5.3 Cleaning and disinfection <\/td>\n<\/tr>\n
36<\/td>\n6.5.4 Maintenance and inspection
6.6 Homogenizers, blenders, mixers and shakers
6.6.1 Homogenizers and blenders <\/td>\n<\/tr>\n
37<\/td>\n6.6.2 Vortex mixers <\/td>\n<\/tr>\n
38<\/td>\n6.7 Stills, deionizers and reverse-osmosis units
6.7.1 Description
6.7.2 Use
6.7.3 Maintenance
6.7.4 Verification
6.8 Separation and concentration equipment
6.8.1 Immunomagnetic separator (IMS) <\/td>\n<\/tr>\n
39<\/td>\n6.8.2 Centrifuge
6.8.3 Filtration systems
6.9 Modified atmosphere equipment
6.9.1 Description
6.9.2 Use <\/td>\n<\/tr>\n
40<\/td>\n6.9.3 Maintenance
6.9.4 Verification
6.10 Other equipment
6.10.1 pH meter <\/td>\n<\/tr>\n
41<\/td>\n6.10.2 Colony-counting device
6.10.3 Timers and timing devices <\/td>\n<\/tr>\n
42<\/td>\n6.10.4 Optical microscope
6.10.5 Glass washers, glassware and other laboratory ware <\/td>\n<\/tr>\n
43<\/td>\n6.10.6 Disposable equipment and consumables <\/td>\n<\/tr>\n
44<\/td>\n6.10.7 Other equipment and software
7.1 Sterilization
7.1.1 General
7.1.2 Sterilization by dry heat
7.1.3 Sterilization by moist heat (steam)
7.2 Decontamination and disinfection
7.2.1 Decontamination of glassware and materials before use
7.2.2 Decontamination of glassware and materials after use <\/td>\n<\/tr>\n
45<\/td>\n7.3 Waste management
7.4 Washing <\/td>\n<\/tr>\n
46<\/td>\n9.1 Sampling techniques and sampling plans
9.1.1 General
9.1.2 Sampling
9.2 Sample transport <\/td>\n<\/tr>\n
47<\/td>\n9.3 Sample receipt
9.4 Sample handling
9.4.1 General <\/td>\n<\/tr>\n
48<\/td>\n9.4.2 Storage before examination
9.4.3 Test portions
9.4.4 Storage of laboratory samples after examination
9.5 Pre-testing of samples <\/td>\n<\/tr>\n
49<\/td>\n10.1 Hygienic precautions during sample preparation and examination
10.1.1 General
10.1.2 Basic precautions
10.1.3 Sample handling <\/td>\n<\/tr>\n
50<\/td>\n10.1.4 Sample handling tools and implements
10.1.5 Spillages
10.1.6 Process controls
10.1.7 Aerosols <\/td>\n<\/tr>\n
51<\/td>\n10.1.8 Molecular methods
10.2 Preparation of initial suspension and dilutions
10.2.1 General
10.2.2 Concentration
11.1 General <\/td>\n<\/tr>\n
52<\/td>\n11.2 Enumeration using a solid medium
11.2.1 General
11.2.2 Pour plate technique <\/td>\n<\/tr>\n
53<\/td>\n11.2.3 Surface plating techniques <\/td>\n<\/tr>\n
54<\/td>\n11.2.4 Enumeration of yeasts and moulds <\/td>\n<\/tr>\n
55<\/td>\n11.2.5 Incubation
11.2.6 Calculation and expression of results obtained with solid culture media <\/td>\n<\/tr>\n
57<\/td>\n11.2.7 Calculations for enumeration methods <\/td>\n<\/tr>\n
64<\/td>\n11.3 Enumeration using liquid media
11.3.1 Principle
11.3.2 General MPN procedure
11.3.3 Limitations of MPN <\/td>\n<\/tr>\n
65<\/td>\n11.3.4 Inoculation procedure
11.3.5 Choice of MPN configuration <\/td>\n<\/tr>\n
66<\/td>\n11.3.6 Incubation
11.3.7 Interpretation and expression of results
11.3.8 Determination of MPN values using MPN calculators <\/td>\n<\/tr>\n
67<\/td>\n11.3.9 Rarity categories
11.4 Estimates of uncertainty of test results <\/td>\n<\/tr>\n
68<\/td>\n12.1 General
12.2 Principle
13.1 General <\/td>\n<\/tr>\n
69<\/td>\n13.2 Preparation of a pure culture
13.3 Confirmation methods
13.3.1 Latex agglutination test
13.3.2 Nucleic acid hybridization or molecular amplification methods <\/td>\n<\/tr>\n
70<\/td>\n13.3.3 Slide agglutination tests
13.4 Identification methods
13.4.1 Biochemical galleries
13.4.2 DNA sequencing <\/td>\n<\/tr>\n
71<\/td>\n13.4.3 Mass spectrometry
14.1 General <\/td>\n<\/tr>\n
72<\/td>\n14.2 Characterization of microorganisms
14.2.1 General
14.2.2 Phenotypic characterization
14.2.3 Molecular characterization
14.3 Selection of control microorganisms <\/td>\n<\/tr>\n
74<\/td>\n16.1 General <\/td>\n<\/tr>\n
75<\/td>\n16.2 Internal quality control
16.2.1 General
16.2.2 Process controls <\/td>\n<\/tr>\n
76<\/td>\n16.2.3 Replicate testing
16.2.4 Spiked samples
16.2.5 IQC assessment using control charts
16.3 External quality assessment <\/td>\n<\/tr>\n
77<\/td>\n17.1 General
17.2 Performance characteristics
17.3 Validation <\/td>\n<\/tr>\n
78<\/td>\n17.4 Verification <\/td>\n<\/tr>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Microbiology of the food chain. General requirements and guidance for microbiological examinations<\/b><\/p>\n\n\n\n\n
Published By<\/td>\nPublication Date<\/td>\nNumber of Pages<\/td>\n<\/tr>\n
BSI<\/b><\/a><\/td>\n2024<\/td>\n92<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"featured_media":464840,"template":"","meta":{"rank_math_lock_modified_date":false,"ep_exclude_from_search":false},"product_cat":[124,2641],"product_tag":[],"class_list":{"0":"post-464830","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-07-100-30","7":"product_cat-bsi","9":"first","10":"instock","11":"sold-individually","12":"shipping-taxable","13":"purchasable","14":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product\/464830","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media\/464840"}],"wp:attachment":[{"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/media?parent=464830"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_cat?post=464830"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/pdfstandards.shop\/wp-json\/wp\/v2\/product_tag?post=464830"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}